7EXG
Crystal structure of D383A mutant from Arabidopsis thaliana complexed with Galactose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-03-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.030, 103.736, 182.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7exf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.410 | 2.050 |
| Rmerge | 0.075 | 0.053 | 0.554 |
| Rmeas | 0.082 | 0.058 | 0.606 |
| Rpim | 0.032 | 0.023 | 0.241 |
| Total number of observations | 714794 | ||
| Number of reflections | 111655 | 11589 | 10589 |
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.3 | 98.6 | 95.4 |
| Redundancy | 6.4 | 5.8 | 5.8 |
| CC(1/2) | 0.996 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.8 | 291.15 | 0.1 M Tris, PEG 2000, PGA |
