7EXF

Crystal structure of wild-type from Arabidopsis thaliana complexed with Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2017-06-13
Detector	RAYONIX MX300-HS
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	94.938, 103.644, 181.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.170
R-factor	0.2109
Rwork	0.208
R-free	0.26690
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.818
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.250
High resolution limit [Å]	2.170	4.670	2.170
Rmerge	0.078	0.044	0.776
Rmeas	0.086	0.049	0.848
Rpim	0.036	0.020	0.338
Total number of observations	568408
Number of reflections	95207	9992	7988
<I/σ(I)>	13.4
Completeness [%]	98.2	98.4	83.7
Redundancy	6	5.1	6
CC(1/2)		0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.8	291.15	Tris, PEG 2000, PGA