7EXB

DfgA-DfgB complex apo 2.4 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.88
Spacegroup name	P 64 2 2
Unit cell lengths	138.970, 138.970, 227.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.100 - 2.400
R-factor	0.179
Rwork	0.178
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bvs
RMSD bond length	0.007
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	1.417
Number of reflections	51290	4359
<I/σ(I)>	27.4	2.8
Completeness [%]	100.0	99.9
Redundancy	36.9	22.8
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl (pH8.5), 1190 mM (NH4)2SO4