7EWZ

Crystal structure of Ebinur Lake virus cap snatching endonuclease (D92A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	40.871, 72.189, 71.868
Unit cell angles	90.00, 90.55, 90.00

Refinement procedure

Resolution	27.050 - 2.050
R-factor	0.2289
Rwork	0.227
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.078	0.061	0.549
Rmeas	0.087	0.069	0.605
Rpim	0.038	0.031	0.249
Total number of observations	130729
Number of reflections	24961	2665	2014
<I/σ(I)>	12.2
Completeness [%]	94.5	98.4	76.5
Redundancy	5.2	5	4.7
CC(1/2)		0.994	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.9	289	PEG 3350