7EWT
The crystal structure of Lysophospholipid acyltransferase LPCAT3 (MOBAT5) in its monomeric and apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.789, 82.261, 116.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.650 - 3.400 |
| R-factor | 0.343 |
| Rwork | 0.343 |
| R-free | 0.34500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 3.450 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 10597 | 1663 |
| <I/σ(I)> | 21.84 | |
| Completeness [%] | 99.3 | |
| Redundancy | 7.2 | |
| CC(1/2) | 0.572 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 289 | PEG 400, 4-Morpholineethanesulfonic acid (MES), Magnesium Acetate, Formamide |
