7EWM

Native crystal structure of S. cerevisiae Csn12 in complex with Thp3 and Sem1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 32 2 1
Unit cell lengths	115.575, 115.575, 126.825
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.290 - 2.900
R-factor	0.2236
Rwork	0.222
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ewf
RMSD bond length	0.006
RMSD bond angle	0.878
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.115	0.106
Number of reflections	22217	1089
<I/σ(I)>	29.77	2.36
Completeness [%]	99.9
Redundancy	19.3
CC(1/2)	0.995	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	12% PEG3350, 100 mM Sodium malonate buffer (pH 5.0) and 3% Methanol