7EW8
Legionella pneumophila effector AnkD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.058, 75.997, 104.814 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 45.207 - 2.594 |
| R-factor | 0.2043 |
| Rwork | 0.201 |
| R-free | 0.26790 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.095 | 0.653 |
| Number of reflections | 32269 | 15131 |
| <I/σ(I)> | 21 | 2.6 |
| Completeness [%] | 92.8 | 98.1 |
| Redundancy | 6.5 | 4.9 |
| CC(1/2) | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | magnesium chloride, Bis Tris, PEG 3350 |
