7EVL
Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 64% GTP/36% GDP and 2 Na+ in a small unit cell
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.166, 79.515, 90.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
Rwork | 0.203 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7evc |
RMSD bond length | 0.009 |
RMSD bond angle | 0.914 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.077 | 0.555 |
Rmeas | 0.083 | 0.622 |
Rpim | 0.031 | 0.273 |
Number of reflections | 16163 | 669 |
<I/σ(I)> | 22.3 | 1.3 |
Completeness [%] | 98.1 | |
Redundancy | 6.7 | |
CC(1/2) | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 25% PEG 1500, 0.1 M MMT, pH 9.0, soaked with 10 mM GTP 1 hr, back soaked 22h with no GTP |