7EVK

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 78% GTP, 22% GDP, Na+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-23
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.364, 92.731, 103.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.200 - 1.750
Rwork	0.154
R-free	0.18260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evc
RMSD bond length	0.009
RMSD bond angle	1.079
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.065	0.433
Rmeas	0.075	0.503
Rpim	0.027	0.253
Number of reflections	39511	1519
<I/σ(I)>	25.1	1.3
Completeness [%]	98.3
Redundancy	6.3
CC(1/2)		0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 3350, 0.2 M sodium acetate 0.1 M Bis-Tris propane, pH 8.5 soaked with 10 mM GTP, 2 mM EGTA, 100 mM KCl (1 h)