7EVI

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 79% GTP, 21% GDP, Na+, Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-23
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.335, 92.220, 102.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.100 - 1.550
Rwork	0.148
R-free	0.17020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evc
RMSD bond length	0.012
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.058	0.245
Rmeas	0.064	0.289
Rpim	0.026	0.149
Number of reflections	55795	2129
<I/σ(I)>	28.4	2.2
Completeness [%]	98.2
Redundancy	5.6
CC(1/2)		0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 3350, 0.2 M sodium acetate 0.1 M Bis-Tris propane, pH 8.5. Crystals were soaked with 5 mM GTP, 2 mM MgCl2 (1 h)