7EVH

Odinarchaeota tubulin H393D mutant, in a psuedo protofilament arrangement, bound to 59% GDP, 41% phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-16
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.300, 93.342, 100.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 2.500
Rwork	0.199
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2r
RMSD bond length	0.005
RMSD bond angle	0.564
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.114	0.544
Rmeas	0.124	0.660
Rpim	0.047	0.364
Number of reflections	13455	526
<I/σ(I)>	14.7	1.5
Completeness [%]	98.0
Redundancy	6.4
CC(1/2)		0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 1500, 0.1 M SPG (2 succinic acid: 7 sodium dihydrogen phosphate), pH 9.0