7EVG
Apo Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a psuedo-protofilament arrangement
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.102, 94.184, 100.755 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.400 - 2.480 |
Rwork | 0.211 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o2r |
RMSD bond length | 0.002 |
RMSD bond angle | 0.532 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.100 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.185 | 1.230 |
Rmeas | 0.201 | 1.340 |
Rpim | 0.078 | 0.516 |
Number of reflections | 14154 | 1525 |
<I/σ(I)> | 8.6 | 1.7 |
Completeness [%] | 99.7 | |
Redundancy | 6.6 | |
CC(1/2) | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 1500, 0.1 M SPG, pH 7.0, soaked with 100 mM phosphate for 2 h |