7EVE

Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 100% GDP and 2 Na+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.130, 88.421, 95.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.500 - 2.000
Rwork	0.211
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7evc
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.114	0.574
Rmeas	0.132	0.674
Rpim	0.065	0.340
Number of reflections	19846	1459
<I/σ(I)>	8.6	2.8
Completeness [%]	85.4
Redundancy	3.5
CC(1/2)		0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 1500, 0.1 M MMT, pH 9.0