7EVB
Odinarchaeota tubulin (OdinTubulin) H393D mutant, in a protofilament arrangement, bound to 77% GTP/23% and 2 Na+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-04-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.327, 92.256, 103.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.410 - 1.620 |
| Rwork | 0.153 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6o2r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.912 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.050 | 0.479 |
| Rmeas | 0.054 | 0.544 |
| Rpim | 0.020 | 0.251 |
| Number of reflections | 48739 | 2085 |
| <I/σ(I)> | 27.6 | 1.6 |
| Completeness [%] | 97.9 | 85.6 |
| Redundancy | 6.5 | 3.7 |
| CC(1/2) | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20% PEG 3350, 0.2 M sodium acetate, 0.1 M Bis-Tris propane, pH 8.5, soaked with 10 mM GTP for 1 h before freezing |
