7EVA

Structure of molecular chaperone SycE of Yersinia enterocolitica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-25
Detector	APEX II CCD
Wavelength(s)	1.5
Spacegroup name	P 43 21 2
Unit cell lengths	58.000, 58.000, 77.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.938 - 2.083
R-factor	0.201
Rwork	0.200
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n5b
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS (1.15.2_3472)
Phasing software	PHASER
Refinement software	PHENIX (1.15.2-3472-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.940	2.140
High resolution limit [Å]	2.080	2.080
Rmerge	0.200	0.278
Number of reflections	8342	8342
<I/σ(I)>	1.51
Completeness [%]	99.7
Redundancy	15.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	100mM MES pH 6.0, 50-80mM Na-Citrate, 5-10% PEG 3350, 100mM NaCl