7EV8

Structure of Human Parainfluenza Virus 3 Unassembled Nucleoprotein in Complex with its viral chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	117.090, 117.090, 143.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.178 - 3.234
Rwork	0.219
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h5q
RMSD bond length	0.008
RMSD bond angle	1.411
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.290
High resolution limit [Å]	3.230	8.750	3.230
Rmerge	0.098	0.032	0.901
Rmeas	0.106	0.035	1.000
Rpim	0.040	0.013	0.422
Total number of observations	56267
Number of reflections	8248	467	395
<I/σ(I)>	9.1
Completeness [%]	99.8	98.1	99.5
Redundancy	6.8	6.1	5.3
CC(1/2)		0.999	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG 6000, Cacodylate, Nickel chloride