7EV4
Crystal structure of the Lon-like protease MtaLonC with S582A mutation in complex with F-b20-Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 1 |
Spacegroup name | P 6 |
Unit cell lengths | 115.597, 115.597, 135.482 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.930 - 2.120 |
R-factor | 0.1825 |
Rwork | 0.180 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fw9 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.799 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.113 | 0.698 |
Number of reflections | 58112 | 5772 |
<I/σ(I)> | 21.5 | 3.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.5 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% isopropanol, 100 mM monosodium phosphate, 100 mM sodium citrate at pH 4.6. |