7EV1

Crystal structure of LI-Cadherin EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	48.900, 79.680, 62.490
Unit cell angles	90.00, 99.02, 90.00

Refinement procedure

Resolution	32.400 - 1.380
R-factor	0.144
Rwork	0.143
R-free	0.18120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zmy
RMSD bond length	0.009
RMSD bond angle	1.542
Data reduction software	MOSFLM (7.1.0)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.310	1.450
High resolution limit [Å]	1.380	1.380
Rmerge	0.088	0.834
Number of reflections	93370	12915
<I/σ(I)>	11.2	1.7
Completeness [%]	96.3	91.7
Redundancy	5.5	5.1
CC(1/2)	0.998	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.2 M Ammonium acetate, 30% 2-propanol, TRIS pH8.5