7EUS
Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) from Cercospora sp. JNU001
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.427, 147.916, 54.163 |
Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
Resolution | 42.620 - 2.300 |
R-factor | 0.1812 |
Rwork | 0.177 |
R-free | 0.21970 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.646 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.290 | 4.950 | 2.300 |
Rmerge | 0.125 | 0.098 | 0.359 |
Rmeas | 0.135 | 0.106 | 0.395 |
Rpim | 0.051 | 0.040 | 0.161 |
Number of reflections | 28264 | 2886 | 2805 |
<I/σ(I)> | 6.1 | ||
Completeness [%] | 98.6 | 98.9 | 98 |
Redundancy | 6.6 | 6.7 | 5.8 |
CC(1/2) | 0.985 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 mM Bis-Tris pH 6.5, 3% (v/v) glycerol, 25% (w/v) PEG 3350 |