7EUM

Crystal structure of apo Nmar_1308 protein at cryogenic temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 32
Unit cell lengths	75.868, 75.868, 207.965
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.687 - 2.150
R-factor	0.2458
Rwork	0.245
R-free	0.28450
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE (XDS)
Phasing software	PHENIX
Refinement software	PHENIX (dev_3318)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.687	29.687	2.280
High resolution limit [Å]	2.150	6.360	2.150
Rmerge	0.127	0.038	1.883
Rmeas	0.134	0.040	1.979
Total number of observations	766012
Number of reflections	72865	2797	11693
<I/σ(I)>	13.38	55.01	1.22
Completeness [%]	99.7	99.4	98.8
Redundancy	10.513	10.366	10.5
CC(1/2)	0.999	0.999	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2M MgCl2, 0.1M sodium citrate tribasic dihydrate (pH 5), 10% w/v PEG