7EUK
Crystal structure of N(omega)-hydroxy-L-arginine hydrolase in complex with L-Orn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 46.392, 46.963, 59.317 |
Unit cell angles | 83.53, 84.71, 70.14 |
Refinement procedure
Resolution | 44.000 - 1.400 |
R-factor | 0.1591 |
Rwork | 0.158 |
R-free | 0.17910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6luh |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.000 | 44.000 | 1.420 |
High resolution limit [Å] | 1.400 | 7.540 | 1.400 |
Rmerge | 0.053 | 0.040 | 0.403 |
Total number of observations | 652069 | 3340 | 27040 |
Number of reflections | 88405 | 591 | 4428 |
<I/σ(I)> | 22.6 | 55.7 | 3.8 |
Completeness [%] | 96.0 | 99.5 | 93.5 |
Redundancy | 7.4 | 5.7 | 6.1 |
CC(1/2) | 0.999 | 0.998 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 298 | PEG 4000, MgCl2 |