7EU9
Crystal structure of the selenomethionine(SeMet)-derived Cas12i1 R-loop complex before target DNA cleavage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 127.064, 141.577, 208.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.630 - 2.350 |
R-factor | 0.2297 |
Rwork | 0.228 |
R-free | 0.26800 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.433 |
Data reduction software | xia2 (0.5.898) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHENIX (1.13-2998) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.140 | 0.969 |
Number of reflections | 78375 | 11343 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 | |
CC(1/2) | 0.997 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium citrate (pH 5.6), 17% (w/v) Polyethylene glycol 3350, 0.1 M sodium citrate |