7EU4
Crystal structure of plant ATG12 complexed with the AIM12 of ATG3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-07-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 64 |
Unit cell lengths | 128.471, 128.471, 163.165 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.960 - 3.200 |
Rwork | 0.238 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wz3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.592 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.960 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 25048 | 1227 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.452 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6~8% (w/v) PEG 3350, 100mM citrate buffer |