7EU4

Crystal structure of plant ATG12 complexed with the AIM12 of ATG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	95
Detector technology	CCD
Collection date	2013-07-05
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 64
Unit cell lengths	128.471, 128.471, 163.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.960 - 3.200
Rwork	0.238
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz3
RMSD bond length	0.011
RMSD bond angle	1.592
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	25048	1227
<I/σ(I)>	7.1
Completeness [%]	99.5
Redundancy	3.4
CC(1/2)		0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6~8% (w/v) PEG 3350, 100mM citrate buffer