7ETX
Crystal structure of bifunctional indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase (TrpC) from corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.681, 90.681, 141.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.740 - 2.100 |
R-factor | 0.1954 |
Rwork | 0.193 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.522 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.083 | 0.061 | 0.345 |
Rmeas | 0.087 | 0.063 | 0.376 |
Rpim | 0.024 | 0.018 | 0.139 |
Total number of observations | 352134 | ||
Number of reflections | 34548 | 1888 | 1664 |
<I/σ(I)> | 13.9 | ||
Completeness [%] | 98.0 | 95.8 | 96.6 |
Redundancy | 10.2 | 12.8 | 5.5 |
CC(1/2) | 0.998 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 0.8M Pottasium sodium tartrate tetrahydrate, 0.1M Sodium HEPES pH 7.5 |