7ET5

Crystal structure of Arabidopsis TEM1 AP2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	37.650, 41.200, 59.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.958 - 1.052
R-factor	0.1641
Rwork	0.164
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gcc
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.090
High resolution limit [Å]	1.050	1.050
Rmerge	0.050	0.791
Rmeas	0.055	0.881
Rpim	0.022	0.378
Number of reflections	42719	3611
<I/σ(I)>	24.2	1.2
Completeness [%]	96.2	83
Redundancy	6.2	4.9
CC(1/2)	1.000	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277.15	0.2 M Li2SO4, 0.1 M Tris pH 8.5, 18% PEG 8000