7ET4
Crystal structure of Arabidopsis TEM1 AP2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.316, 135.155, 66.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.524 - 2.700 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcc |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.140 | 0.819 |
Rmeas | 0.160 | 0.947 |
Rpim | 0.076 | 0.464 |
Number of reflections | 27364 | 2704 |
<I/σ(I)> | 7.4 | 1.25 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.3 | 4.1 |
CC(1/2) | 0.984 | 0.499 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.2 M NH4Cl, 0.1 M NaAc pH 5.0, 20% PEG 6000 |