7ESW
Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-02-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.290, 48.720, 56.200 |
Unit cell angles | 90.00, 112.35, 90.00 |
Refinement procedure
Resolution | 31.950 - 1.250 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.17450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cwj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.054 | 0.497 |
Rmeas | 0.060 | 0.554 |
Rpim | 0.025 | 0.240 |
Number of reflections | 64807 | 3121 |
<I/σ(I)> | 17 | |
Completeness [%] | 99.0 | |
Redundancy | 5.5 | |
CC(1/2) | 0.997 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.9M Sodium malonate pH 6.5, 5% Glycerol |