7ESS
Structure-guided studies of the Holliday junction resolvase RuvX provide novel insights into ATP-stimulated cleavage of branched DNA and RNA substrates
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-03-28 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.490, 42.580, 58.110 |
Unit cell angles | 90.00, 114.45, 90.00 |
Refinement procedure
Resolution | 52.950 - 1.930 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vhx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.484 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.477 | 53.477 | 2.040 |
High resolution limit [Å] | 1.930 | 6.110 | 1.930 |
Rmerge | 0.036 | 0.325 | |
Rmeas | 0.075 | 0.042 | 0.368 |
Rpim | 0.034 | 0.021 | 0.171 |
Number of reflections | 19756 | 675 | 2772 |
<I/σ(I)> | 13.2 | 9.5 | 2.2 |
Completeness [%] | 99.3 | 99.9 | 96.9 |
Redundancy | 4.7 | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 300 | 0.1 M Bis-Tris propane, pH 7.0 and 2.4 M sodium malonate, pH 7.0 |