7ESC
FmnB complexed with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.875, 67.872, 97.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.460 - 2.201 |
| Rwork | 0.245 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7esa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.415 |
| Data reduction software | xia2 (0.7.7) |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.460 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | |
| Number of reflections | 16541 | 1618 |
| <I/σ(I)> | 16.63 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.995 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286 | 0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350 |
