7ESB
FmnB complexed with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.089, 67.294, 86.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.370 - 1.700 |
| Rwork | 0.171 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7esa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.874 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.370 | 1.764 |
| High resolution limit [Å] | 1.700 | 1.703 |
| Rmerge | 0.200 | |
| Number of reflections | 42584 | 3873 |
| <I/σ(I)> | 31.76 | |
| Completeness [%] | 98.8 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.980 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.2M sodium formate, 20% w/v Polyethylene glycol 3350 |
