7ESA
the complex structure of flavin transferase FmnB complexed with FAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.089, 67.294, 86.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.280 - 1.800 |
Rwork | 0.194 |
R-free | 0.22270 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.449 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | CRANK2 |
Refinement software | PHENIX (v1.17) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.280 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.119 | |
Number of reflections | 36579 | 3603 |
<I/σ(I)> | 48.17 | |
Completeness [%] | 100.0 | |
Redundancy | 12.9 | |
CC(1/2) | 0.971 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 286 | 0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350 |