7ER6
Crystal structure of human Biliverdin IX-beta reductase B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.871, 117.440, 41.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.720 - 1.600 |
R-factor | 0.1632 |
Rwork | 0.162 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1he5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.115 |
Data scaling software | HKL-2000 (1.16_3549) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 1.653 |
High resolution limit [Å] | 1.596 | 1.596 |
Rmerge | 0.098 | 0.374 |
Number of reflections | 53419 | 5047 |
<I/σ(I)> | 13.85 | |
Completeness [%] | 98.6 | |
Redundancy | 7.8 | |
CC(1/2) | 0.971 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 1.9M-2.0M Ammonium sulfate, 0.1M Bis-tris (pH 6.5) |