7EQZ
Crystal structure of Carboxypeptidase B complexed with Potato Carboxypeptidase Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-09-09 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.451, 87.208, 49.069 |
Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
Resolution | 31.890 - 2.200 |
R-factor | 0.1586 |
Rwork | 0.154 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cpa |
RMSD bond length | 0.002 |
RMSD bond angle | 0.502 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.890 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 15975 | 1009 |
<I/σ(I)> | 48.29 | |
Completeness [%] | 96.6 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.6M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5, 0.1M Sodium chloride |