7EQX
Crystal structure of an Aedes aegypti procarboxypeptidase B1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-10-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.797, 74.436, 83.108 |
Unit cell angles | 90.00, 119.08, 90.00 |
Refinement procedure
Resolution | 33.630 - 2.080 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.626 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.160 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 44087 | 2867 |
<I/σ(I)> | 27.74 | |
Completeness [%] | 98.4 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulphate, 0.1M sodium cacodylate trihydrate pH 6.5, 30% w/v PEG 8000 |