7EQM
Apo Truncated VhChiP (Delta 1-19)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.244, 151.388, 95.827 |
Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
Resolution | 20.003 - 2.500 |
R-factor | 0.180844482218 |
Rwork | 0.178 |
R-free | 0.22517 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mdo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.857 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.003 | 7.980 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.119 | |
Rpim | 0.061 | |
Number of reflections | 43095 | 43095 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 89.4 | |
Redundancy | 3.1 | |
CC(1/2) | 0.868 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 292 | 0.1 M Lithium sulfate, 0.1 M ADA pH 5.2, and 26% v/v PEG 400 |