7EQJ

crystal structure of E. coli Valine tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	53.541, 33.059, 131.797
Unit cell angles	90.00, 98.15, 90.00

Refinement procedure

Resolution	29.488 - 2.043
R-factor	0.2061
Rwork	0.204
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zw4
RMSD bond length	0.005
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.090
High resolution limit [Å]	2.043	5.550	2.050
Rmerge	0.099	0.052	0.827
Rmeas	0.108	0.057	0.908
Rpim	0.041	0.021	0.366
Number of reflections	28804	1571	1309
<I/σ(I)>	4.8
Completeness [%]	97.4	99.1	88.9
Redundancy	6.4	6.7	5.1
CC(1/2)		0.998	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.05M Magnesium chloride hexahydrate 0.1M HEPES pH 7.5 30%(v/v) polyethylene glycol monomethyl ether 550 30%(v/v) 2-propanol