7EQH

Crystal structure of Arabidopsis GUN2/HO1 in complex with heme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.622, 84.759, 92.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.750 - 2.200
R-factor	0.2098
Rwork	0.208
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.131	0.068
Rmeas	0.142	0.075
Rpim	0.058	0.030	0.587
Total number of observations	197106
Number of reflections	27162	2857	2686
<I/σ(I)>	5.1
Completeness [%]	99.8	98.2	100
Redundancy	7.3	6.6	7.4
CC(1/2)		0.995	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	0.17 M Ammonium acetate, 0.085 M Sodium acetate trihydrate pH 4.6, 25.5% w/v Polyethylene glycol 4000, 15% v/v Glycerol