7EQE
Crystal Structure of a transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97899 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.790, 53.130, 102.940 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 47.943 - 2.399 |
R-factor | 0.2418 |
Rwork | 0.240 |
R-free | 0.26740 |
Structure solution method | MAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.943 | 2.540 |
High resolution limit [Å] | 2.399 | 2.399 |
Number of reflections | 65417 | 10421 |
<I/σ(I)> | 7.39 | |
Completeness [%] | 99.4 | |
Redundancy | 3.51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | HEPES-NaOH, polyethylene glycol 4000, isopropanol |