7EQC

Crystal structure of the mini-centralspindlin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	64.756, 65.406, 75.789
Unit cell angles	96.42, 92.24, 105.03

Refinement procedure

Resolution	41.653 - 2.500
R-factor	0.2397
Rwork	0.238
R-free	0.27740
Structure solution method	SIRAS
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.500	2.500
Number of reflections	39530	3953
<I/σ(I)>	21.076
Completeness [%]	96.0
Redundancy	3.7
CC(1/2)	0.829	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	8-10% PEG3350, 100mM NaAc 5.0, 100mM tacsimate 5.0, 5mM DTT