7EQB

Crystal structure of the dimerization domain of ZEN-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	116.542, 116.542, 47.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.937 - 2.103
R-factor	0.2324
Rwork	0.231
R-free	0.26860
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	19067	1695
<I/σ(I)>	16.949
Completeness [%]	98.2
Redundancy	4.8
CC(1/2)	0.522	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	16% PEG2K, 200mM MgSO4, 5mM DTT