7EQ7

Co-Crystal Structure Analysis of Annexin A2 and 5alpha-EAL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	49.223, 58.534, 63.122
Unit cell angles	90.00, 102.86, 90.00

Refinement procedure

Resolution	24.257 - 2.111
R-factor	0.2012
Rwork	0.199
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.540	61.540	2.230
High resolution limit [Å]	2.110	6.680	2.110
Rmerge	0.031	0.020	0.326
Rmeas	0.034	0.022	0.389
Rpim	0.014	0.009	0.205
Total number of observations	96130	4002	5348
Number of reflections	16783	670	1597
<I/σ(I)>	27.1	66.9	2.7
Completeness [%]	83.0	98.9	55.6
Redundancy	5.7	6	3.3
CC(1/2)	0.999	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	100 mM Tris, 20% PEG3350, 0.2 M sodium acetate