7EQ5

Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/W215G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2019-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 65
Unit cell lengths	176.483, 176.483, 63.603
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.890 - 2.600
R-factor	0.1835
Rwork	0.182
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxn
RMSD bond length	0.002
RMSD bond angle	0.509
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rpim	0.029	0.074
Number of reflections	35219	1748
<I/σ(I)>	23.88
Completeness [%]	100.0
Redundancy	17.2
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M HEPES sodium pH 7.0, 1.5 M Lithium sulfate monohydrate