7EQ5
Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/W215G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100.15 |
Detector technology | PIXEL |
Collection date | 2019-12-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 65 |
Unit cell lengths | 176.483, 176.483, 63.603 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.890 - 2.600 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dxn |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rpim | 0.029 | 0.074 |
Number of reflections | 35219 | 1748 |
<I/σ(I)> | 23.88 | |
Completeness [%] | 100.0 | |
Redundancy | 17.2 | |
CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1 M HEPES sodium pH 7.0, 1.5 M Lithium sulfate monohydrate |