7EQ4
Crystal Structure of the N-terminus of Nonstructural protein 1 from SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2020-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9822 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.605, 36.605, 140.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.460 - 1.250 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hsx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.899 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.310 | 8.000 |
| High resolution limit [Å] | 1.150 | 1.250 |
| Number of reflections | 50465 | 27553 |
| <I/σ(I)> | 36.33 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 0.2 M Lithium sulfate monohydrate, 0.1 M Glycine pH 9.5, 30% w/v Polyethylene glycol 4000 |
