7EQ4

Crystal Structure of the N-terminus of Nonstructural protein 1 from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	CCD
Collection date	2020-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9822
Spacegroup name	P 43 21 2
Unit cell lengths	36.605, 36.605, 140.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.460 - 1.250
R-factor	0.165
Rwork	0.163
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hsx
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (7.1.002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.310	8.000
High resolution limit [Å]	1.150	1.250
Number of reflections	50465	27553
<I/σ(I)>	36.33
Completeness [%]	99.9
Redundancy	14
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	0.2 M Lithium sulfate monohydrate, 0.1 M Glycine pH 9.5, 30% w/v Polyethylene glycol 4000