7EQ3

Apo diabody form of CRH2-directed antibody 9F8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-09-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5406
Spacegroup name	P 31 2 1
Unit cell lengths	106.806, 106.806, 50.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.250 - 2.870
R-factor	0.2384
Rwork	0.238
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v6o
Data scaling software	HKL-2000
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.250	2.980
High resolution limit [Å]	2.870	2.870
Number of reflections	7564	621
<I/σ(I)>	9.7
Completeness [%]	91.3
Redundancy	8.9
CC(1/2)	0.750	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris pH 8.0, 0.2M Ammonium acetate and 16% PEG 10000