7EPK
Crystal Structure of Signal Recognition Particle 54 kDa protein (SRP54) from Aeropyrum pernix K1 in Complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-01-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 82.498, 82.498, 140.805 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.280 - 2.700 |
R-factor | 0.2256 |
Rwork | 0.224 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kl4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.592 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BUCCANEER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.280 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 15103 | 1400 |
<I/σ(I)> | 20.51 | 2.29 |
Completeness [%] | 98.4 | 92.3 |
Redundancy | 6.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200 mM KH2PO4, 20% (w/v) PEG 3350 |