7EP4
Crystal structure of ZER1 bound to GFLH degron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2020-12-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979191 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.940, 70.150, 148.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.430 - 2.070 |
Rwork | 0.179 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ep0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.842 |
Data reduction software | XDS (0.7.4) |
Data scaling software | XDS (0.7.4) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.430 | 2.120 |
High resolution limit [Å] | 2.066 | 2.070 |
Rmerge | 0.258 | 0.627 |
Number of reflections | 38879 | 2661 |
<I/σ(I)> | 19.5 | 8.5 |
Completeness [%] | 99.3 | |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium formate, 20% (wt/vol) Polyethylene glycol 3,350 |