7EP3

Crystal structure of ZER1 bound to GAGN degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-12-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.979191
Spacegroup name	C 2 2 21
Unit cell lengths	67.970, 120.420, 68.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.323 - 1.513
Rwork	0.219
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
RMSD bond length	0.006
RMSD bond angle	0.752
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.190	1.550
High resolution limit [Å]	1.510	1.510
Rmerge	0.038	0.440
Number of reflections	43366	2864
<I/σ(I)>	30.6	2.8
Completeness [%]	98.8
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium formate, 23% (wt/vol) Polyethylene glycol 3,350, 0.033% (wt/vol) Anthrone, 0.033% (wt/vol) Congo Red, 0.033% (wt/vol) N-(2-Acetamido)-2-aminoethanesulfonic acid, 0.002 M HEPES sodium pH 6.8