7EP2

Crystal structure of ZYG11B bound to GGFN degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-05-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	91.360, 102.320, 164.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.280 - 2.380
Rwork	0.197
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
RMSD bond length	0.008
RMSD bond angle	0.904
Data reduction software	XDS (0.7.4)
Data scaling software	XDS (0.7.4)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.320	2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.110	1.536
Number of reflections	62538	4544
<I/σ(I)>	15.8
Completeness [%]	99.9
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium Sulfate, 0.1 M HEPES pH 7.5, 25% (wt/vol) Polyethylene glycol 3,350