7EOZ
The structure of rice Defective Pollen Wall (DPW) in the complex with its cofactor NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-03-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 61 |
Unit cell lengths | 222.814, 222.814, 114.024 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.450 - 3.400 |
R-factor | 0.1938 |
Rwork | 0.191 |
R-free | 0.23960 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 2.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
Rmerge | 0.136 | 0.057 | 0.788 |
Total number of observations | 540754 | ||
Number of reflections | 48784 | 2422 | 2411 |
<I/σ(I)> | 16 | ||
Completeness [%] | 99.8 | 95.7 | 100 |
Redundancy | 11.1 | 9.4 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 2.8 M sodium formate, pH 7.0, 0.1 M magnesium chloride hexahydrate, 0.1 M HEPES, pH 7.5, 5% (w/v) PEG 3350 |