7EOX
Protease structure from Euphorbia resinifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.673, 112.880, 201.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.010 - 1.700 |
R-factor | 0.20434 |
Rwork | 0.203 |
R-free | 0.23667 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.621 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.160 | 64.160 | 1.730 |
High resolution limit [Å] | 1.700 | 9.310 | 1.700 |
Rmerge | 0.116 | 0.031 | 2.683 |
Rmeas | 0.141 | 0.038 | 3.278 |
Rpim | 0.080 | 0.021 | 1.853 |
Number of reflections | 168106 | 1029 | 8027 |
<I/σ(I)> | 9.7 | ||
Completeness [%] | 99.0 | ||
Redundancy | 5.8 | 5.8 | 6 |
CC(1/2) | 0.995 | 0.972 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M sodium idodide (0.2 M), 0.1M Bis-Tris propane pH 8.5, 20% W/V PEG 3350 |